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rabbit anti puma bbc3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti puma bbc3
    Rabbit Anti Puma Bbc3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+puma+bbc3/Puma+Rabbit+mAb/pmc12856326-8-0-4
    Average 95 stars, based on 189 article reviews
    rabbit anti puma bbc3 - by Bioz Stars, 2026-11
    95/100 stars

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    Cell-Signaling:

    Article Title: Aberrant CDK4/6-driven cell-cycle reentry drives neuronal loss and defines a therapeutic target in C9orf72 ALS/FTD
    Article Snippet: Rabbit anti- C9orf72 , Cell Signaling , Cat# 64196; RRID:AB_2833080. .. Rabbit anti-PUMA (BBC3) , Cell Signaling , Cat# 12450; RRID:AB_2797920. .. Rabbit anti-PSD95 , Cell Signaling , Cat# 3450S; RRID:AB_2292883.



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    Pharmacodynamic analysis of eribulin, irinotecan and the combination in ES-4 xenografts. (A) Immunoblotting of three independent ES-4 tumor lysates for markers of mitotic arrest (P-S10-histone H3), DNA damage, (P-S139-H2A.X; <t>P-S345-Chk1;</t> P-T68-Chk2; RRM2B, ribonucleotide reductase regulatory TP53-inducible subunit M2B), TP53-regulated genes (TP53; cyclin dependent kinase inhibitor 1A, p21; MDM2; PUMA) and apoptosis (full length and cleaved caspase 3; cleaved PARP). GAPDH was used as a loading control. (B) Immunohistochemistry for TP53 in ES-4 xenografts treated with eribulin, irinotecan or the combination. Tumors were excised and processed at 24h and 144h after the first drug dose. (C) Quantification of TP53 staining in ES-4 xenografts. *p < 0.033, **p < 0.002, ***p < 0.001 compared to control; #p < 0.033 compared to eribulin + irinotecan at 24 h; $p < 0.033, $$p < 0.002 compared to eribulin + irinotecan at 144h, two-way ANOVA with Tukey’s post-hoc test. ERB, eribulin; IRN, irinotecan; ERB+IRN, combination treatment.
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    Pharmacodynamic analysis of eribulin, irinotecan and the combination in ES-4 xenografts. (A) Immunoblotting of three independent ES-4 tumor lysates for markers of mitotic arrest (P-S10-histone H3), DNA damage, (P-S139-H2A.X; P-S345-Chk1; P-T68-Chk2; RRM2B, ribonucleotide reductase regulatory TP53-inducible subunit M2B), TP53-regulated genes (TP53; cyclin dependent kinase inhibitor 1A, p21; MDM2; PUMA) and apoptosis (full length and cleaved caspase 3; cleaved PARP). GAPDH was used as a loading control. (B) Immunohistochemistry for TP53 in ES-4 xenografts treated with eribulin, irinotecan or the combination. Tumors were excised and processed at 24h and 144h after the first drug dose. (C) Quantification of TP53 staining in ES-4 xenografts. *p < 0.033, **p < 0.002, ***p < 0.001 compared to control; #p < 0.033 compared to eribulin + irinotecan at 24 h; $p < 0.033, $$p < 0.002 compared to eribulin + irinotecan at 144h, two-way ANOVA with Tukey’s post-hoc test. ERB, eribulin; IRN, irinotecan; ERB+IRN, combination treatment.

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Evaluation of Eribulin Combined with Irinotecan for Treatment of Pediatric Cancer Xenografts.

    doi: 10.1158/1078-0432.CCR-19-1822

    Figure Lengend Snippet: Pharmacodynamic analysis of eribulin, irinotecan and the combination in ES-4 xenografts. (A) Immunoblotting of three independent ES-4 tumor lysates for markers of mitotic arrest (P-S10-histone H3), DNA damage, (P-S139-H2A.X; P-S345-Chk1; P-T68-Chk2; RRM2B, ribonucleotide reductase regulatory TP53-inducible subunit M2B), TP53-regulated genes (TP53; cyclin dependent kinase inhibitor 1A, p21; MDM2; PUMA) and apoptosis (full length and cleaved caspase 3; cleaved PARP). GAPDH was used as a loading control. (B) Immunohistochemistry for TP53 in ES-4 xenografts treated with eribulin, irinotecan or the combination. Tumors were excised and processed at 24h and 144h after the first drug dose. (C) Quantification of TP53 staining in ES-4 xenografts. *p < 0.033, **p < 0.002, ***p < 0.001 compared to control; #p < 0.033 compared to eribulin + irinotecan at 24 h; $p < 0.033, $$p < 0.002 compared to eribulin + irinotecan at 144h, two-way ANOVA with Tukey’s post-hoc test. ERB, eribulin; IRN, irinotecan; ERB+IRN, combination treatment.

    Article Snippet: Proteins were transferred to Immobilon-FL PVDF membranes (MilliporeSigma) and probed with antibodies for cleaved PARP (Cell Signaling #5625), phospho-S139-H2A.X (#9718), TP53 (#48818), CDKN1A/p21 (#2947), MDM2 (#86934), PUMA/BBC3 (#12450) phospho-S345-Chk1 (#2348), Chk1 (#2360), phospho-T68-Chk2 (#2197), Chk2 (#3440), full length/cleaved caspase 3 (#9668), cleaved caspase 3 (#9664), phospho-S10-histone H3 (#53348), histone H3 (#4499), GAPDH (#2118) or RRM2B/p53R2 (Invitrogen #PA5–19970).

    Techniques: Western Blot, Immunohistochemistry, Staining